OBJECTIVES: This study compared the effects of three carbohydrate-hydration strategies on blood glucose concentration, exercise performance and hydration status throughout simulated soccer match-play. DESIGN: A randomized, double-blind and cross-over study design was employed. METHODS: After familiarization, 14 recreational soccer players completed the soccer match simulation on three separate occasions. Participants consumed equal volumes of 9.6% carbohydrate-caffeine-electrolyte (~6mg/kg BW caffeine) solution with carbohydrate-electrolyte gels (H-CHO), 5.6% carbohydrate-electrolyte solution with electrolyte gels (CHO) or electrolyte solution and electrolyte gels (PL). Blood samples were taken at rest, immediately before exercise and every 15min during exercise (first half: 15, 30, 45min; second half: 60, 75, 90min). RESULTS: Supplementation influenced blood glucose concentration (time×treatment interaction: p<0.001); however, none of the supplementation regimes were effective in preventing a drop in blood glucose at 60min. Mean sprint speed was 3±1% faster in H-CHO when compared with PL (treatment: p=0.047). Supplementation caused a 2.3±0.5% increase in plasma osmolality in H-CHO (p<0.001) without change in CHO or PL. Similarly, mean sodium concentrations were 2.1±0.4% higher in H-CHO when compared with PL (p=0.006). CONCLUSIONS: Combining high carbohydrate availability with caffeine resulted in improved sprint performance and elevated blood glucose concentrations throughout the first half and at 90min of exercise; however, this supplementation strategy negatively influenced hydration status when compared with 5.6% carbohydrate-electrolyte and electrolyte solutions.